Designing Elisa Method for Measuring the Level of Anti-streptokinase Antibody
نویسندگان
چکیده
Objectives: Streptokinase, a protein produced by group C streptococci, is prescribed as a thrombosis treatment for the patients who suffer from acute myocardial infarction. Antibodies that are produced to neutralize streptokinase are challenges in treatment of MI patient particularly those who experience the attack for the second time. The purpose of this study was to introduce a simple enzyme immunoassay for determination of antistreptokinase antibodies in plasma of patients. Methodology: For designing the standard method of Indirect Immunoassay or ELISA, commercially available reagents have been used for the assay, which are calibrated with serums of healthy individuals as control group and patients with acute myocardial as experimental group. Level of anti-streptokinase antibodies of treated patients with streptokinase and healthy voluntaries were assayed after 0, 1 and 6 months and results were analysed with statistical
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